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Affinity Biosciences
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Wuhan Sanying Biotechnology
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: NLRC5 Deficiency Reduces LPS-Induced Microglial Activation via Inhibition of NF-κB Signaling and Ameliorates Mice’s Depressive-like Behavior
doi: 10.3390/ijms241713265
Figure Lengend Snippet: NLRC5 regulates microglial activation via promoting phosphorylation of IKKα/β. ( A ) Representative Western blot image of p-IKKα/β, IKKα/β, p-IκB and IκB in BV2-shCtrl and BV2-sh Nlrc5 cells treated with LPS (10 ng/mL) for different times. Bars represent the relative fold change of p-IKKα/β to IKKα/β ( B ) and p-IκB to IκB ( C ) (n = 5 for each group). ( D ) Representative Western blot image of p-TAK1 and TAK1 in BV2-shCtrl and BV2-sh Nlrc5 cells treated with LPS. ( E ) Bars represent the relative fold change of p-TAK1 to TAK1 (n = 5 for each group). ( F ) Anti-NLRC5 was used to precipitate IKKα/β from BV2-shCtrl and BV2-sh Nlrc5 cell extracts. P-IKKα/β and IKKα/β were subsequently determined by Western blot analysis. ( G ) Representative Western blot image of p-IKKα/β, IKKα/β, p-IκB and IκB in RAW264.7-shCtrl and RAW264.7-sh Nlrc5 cells treated with LPS. Bars represent the relative fold change of p-IKKα/β to IKKα/β. ( H ) and p-IκB to IκB ( I ) (n = 5 for each group). All data were presented as mean ± SEM (( B ), LPS: F (3, 32) = 11.51, p < 0.001; NLRC5: F (1, 32) = 15.95, p < 0.001; Interaction: F (3, 32) = 3.201, p < 0.05; ( C ), LPS: F (3, 32) = 8.843, p < 0.001; NLRC5: F (1, 32) = 9.209, p < 0.01; Interaction: F (3, 32) = 4.286, p < 0.05; ( E ), LPS: F (3, 32) = 5.683, p < 0.01; NLRC5: F (1, 32) = 0.3470, p = 0.5600; Interaction: F (3, 32) = 0.1702, p = 0.9157; ( H ), LPS: (3, 32) = 78.14, p < 0.001; NLRC5: F (1, 32) = 8.092, p < 0.01; Interaction: F (3, 32) = 6.471, p < 0.01; ( I) , LPS: (3, 32) = 47.36, p < 0.001; NLRC5: F (1, 32) = 6.760, p < 0.05; Interaction: F (3, 32) = 3.143, p < 0.05). A two-way ANOVA was used to determine the statistical significance of the two groups. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001. ns: no significant difference. p-IKKα/β: phosphorylated IKKα/β; p-IκB: phosphorylated IκB; p-TAK1: phosphorylated TAK1.
Article Snippet: The corresponding antibodies to the protein of interest for immunoblotting included p-IKKα/β (Cell Signaling Technology, Danvers, MA, USA, 2697, 1:1000 dilution), p-I k B (Cell Signaling Technology, 9246, 1:1000 dilution), IKKα/β (Abcam, Cambridge, MA, USA, ab178870, 1:1000 dilution), I k B (Cell Signaling Technology, 9242, 1:1000 dilution), p65 (BD Bioscience, San Jose, CA, USA, 610868, 1:1000 dilution), NLRC5 (Abcam, ab105411, 1:500 dilution),
Techniques: Activation Assay, Phospho-proteomics, Western Blot
Journal: iScience
Article Title: Tripartite motif 38 attenuates cardiac fibrosis after myocardial infarction by suppressing TAK1 activation via TAB2/3 degradation
doi: 10.1016/j.isci.2022.104780
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Protease Inhibitor, Modification, Bicinchoninic Acid Protein Assay, Staining, Software